Identifying potential threats to soil biodiversity.

A decline in soil biodiversity is generally considered to be the reduction of forms of life living in soils, both in terms of quantity and variety. Where soil biodiversity decline occurs, it can significantly affect the soils' ability to function, respond to perturbations and recover from a disturbance. Several soil threats have been identified as having negative effects on soil biodiversity, including human intensive exploitation, land-use change and soil organic matter decline. In this review we consider what we mean by soil biodiversity, and why it is important to monitor. After a thorough review of the literature identified on a Web of Science search concerning threats to soil biodiversity (topic search: threat* "soil biodiversity"), we compiled a table of biodiversity threats considered in each paper including climate change, land use change, intensive human exploitation, decline in soil health or plastic; followed by detailed listings of threats studied. This we compared to a previously published expert assessment of threats to soil biodiversity. In addition, we identified emerging threats, particularly microplastics, in the 10 years following these knowledge based rankings. We found that many soil biodiversity studies do not focus on biodiversity sensu stricto, rather these studies examined either changes in abundance and/or diversity of individual groups of soil biota, instead of soil biodiversity as a whole, encompassing all levels of the soil food web. This highlights the complexity of soil biodiversity which is often impractical to assess in all but the largest studies. Published global scientific activity was only partially related to the threats identified by the expert panel assessment. The number of threats and the priority given to the threats (by number of publications) were quite different, indicating a disparity between research actions versus perceived threats. The lack of research effort in key areas of high priority in the threats to soil biodiversity are a concerning finding and requires some consideration and debate in the research community.

Options for Improved Phosphorus Cycling and Use in Agriculture at the Field and Regional Scales.

Soil phosphorus (P) cycling in agroecosystems is highly complex, with many chemical, physical, and biological processes affecting the availability of P to plants. Traditionally, P fertilizer recommendations have been made using an insurance-based approach, which has resulted in the accumulation of P in many intensively managed agricultural soils worldwide and contributed to the widespread water quality issue of eutrophication. To mitigate further environmental degradation and because future P fertilizer supplies are threatened due to finite phosphate rock resources and associated geopolitical and quality issues, there is an immediate need to increase P use efficiency (PUE) in agroecosystems. Through cultivar selection and improved cropping system design, contemporary research suggests that sufficient crop yields could be maintained at reduced soil test P (STP) concentrations. In addition, more efficient P cycling at the field scale can be achieved through agroecosystem management that increases soil organic matter and organic P mineralization and optimizes arbuscular mycorrhizal fungi (AMF) symbioses. This review paper provides a perspective on how agriculture has the potential to utilize plant and microbial traits to improve PUE at the field scale and accordingly, maintain crop yields at lower STP concentrations. It also links with the need to tighten the P cycle at the regional scale, including a discussion of P recovery and recycling technologies, with a particular focus on the use of struvite as a recycled P fertilizer. Guidance on directions for future research is provided.

Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation.

In soils, phosphorus (P) exists in numerous organic and inorganic forms. However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is frequently limited by P availability. To overcome this problem, rock phosphate fertilisers are heavily applied, often with negative environmental and socio-economic consequences. The organic P fraction of soil contains phospholipids that are rapidly degraded resulting in the release of bioavailable Pi. However, the mechanisms behind this process remain unknown. We identified and experimentally confirmed the function of two secreted glycerolphosphodiesterases, GlpQI and GlpQII, found in Pseudomonas stutzeri DSM4166 and Pseudomonas fluorescens SBW25, respectively. A series of co-cultivation experiments revealed that in these Pseudomonas strains, cleavage of glycerolphosphorylcholine and its breakdown product G3P occurs extracellularly allowing other bacteria to benefit from this metabolism. Analyses of metagenomic and metatranscriptomic datasets revealed that this trait is widespread among soil bacteria with Actinobacteria and Proteobacteria, specifically Betaproteobacteria and Gammaproteobacteria, the likely major players.